As part of the $21 million Termeil Creek Princes Highway upgrade fauna crossings have been built to provide safe passage for wildlife.
Two canopy rope bridges and several glider poles have been installed to connect either side of the road.
These rope bridges will be monitored for three years to assess their use by wildlife.
The fauna underpass at the northern end of the project has been designed to provide a safe crossing for kangaroos, wallabies, bandicoots and echidnas.
A fauna fence has been built on both sides to funnel the animals towards the underpass.
Glider experts from the Southern Cross University (SCU) were engaged throughout the project to advise on the design, location and height of canopy rope bridges and glider poles.
Research from SCU and the Australian Research Centre for Urban Ecology has shown canopy rope bridges and glider poles have been successful in helping animals including the yellow-bellied and squirrel gliders safely cross both the Hume and Pacific Highways.
In addition, 86 nest boxes have been installed to compensate for the loss of 36 trees.
These nest boxes are designed to provide potential habitat for some species including microbats, gliders, possums and birds.
These will be monitored for five years.